ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of peroxisome proliferators-activated receptor (PPAR) in human glioma tissue and its influence on tumor growth.</p><p><b>METHODS</b>Expression of PPAR mRNA in glioma tissue was determined by real-time reverse transcription polymerase chain reaction (RT-PCR). Subsequently, MTT (3-(4, 5)-dimethylthiahiazo(-z-y1)-3, 5-di-phenytetrazoliumromide) assay, flow cytometry, reactive oxygen species assay kit and Western blotting were used to assay U87 cells with agonist activity of PPAR.</p><p><b>RESULTS</b>The data demonstrated that the expression of PPAR in glioma was low and negatively correlated with its pathological grade. Activation of PPAR suppresses tumor cell proliferation, delays the cell cycle at G1 phrase, and induces apoptosis and accumulation of reactive oxygen species (ROS) in U87 cells.</p><p><b>CONCLUSION</b>The expression of PPAR mRNA in human glioma was low. PPAR protein plays a critical role in the progression of glioma via the PPAR signal pathway.</p>
Subject(s)
Humans , Apoptosis , Cell Cycle Checkpoints , Cell Line, Tumor , Cell Proliferation , Gene Expression , Glioma , Genetics , Metabolism , PPAR alpha , Genetics , Metabolism , Signal TransductionABSTRACT
<p><b>OBJECTIVE</b>To study the expression of FOS protein in human glioma tissues and its effect on tumor growth.</p><p><b>METHODS</b>FOS protein expression in glioma tissues was determined with immunohistochemical (IHC) staining. Subsequently, 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, flow cytometry, transwell invasion and Western blotting were used to assay U87 and U251 cells with reduced FOS expression.</p><p><b>RESULTS</b>The expression of FOS in glioma was increased and strongly correlated with its pathological grade. Abrogating expression of FOS has suppressed proliferation and invasion, and delayed cell cycle at G1 phrase for both U87 and U251 cells.</p><p><b>CONCLUSION</b>The expression of FOS protein in human glioma was strong. FOS protein probably plays a critical role in the progression of gliomas.</p>
Subject(s)
Humans , Cell Line, Tumor , Cell Movement , Genetics , Cell Proliferation , Cyclin D1 , Genetics , Metabolism , Gene Expression , Glioma , Genetics , Metabolism , Pathology , Matrix Metalloproteinase 9 , Genetics , Metabolism , Neoplasm Grading , Proto-Oncogene Proteins c-fos , Genetics , Metabolism , RNA InterferenceABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanism of U87 cell apoptosis induced by inhibiting miR-21 expression.</p><p><b>METHODS</b>Antisense oligonucleotides of miR-21 were chemically synthesized and transfected into U87 cells. The apoptosis, proliferation, and invasion of the cells were evaluated. The relationship between miR-21 and PTEN or caspase was identified by bioinformatics and Western blot.</p><p><b>RESULTS</b>Inhibiting miR-21 expression led to U87 cell growth suppression, apoptosis induction, invasion reduction, caspase-3 activity elevation and caspase-9 activation, but did not affect PTEN and caspase-8 expression.</p><p><b>CONCLUSION</b>miR-21 may function as an antiapoptotic miRNA in U87 cells. Inhibiting miR-21 expression could induce U87 cell apoptosis via caspase-9 and 3 activation, but not PTEN activation.</p>
Subject(s)
Animals , Humans , Apoptosis , Genetics , Caspases , Metabolism , Cell Line, Tumor , Cell Proliferation , Enzyme Activation , Genetics , Gene Expression Regulation, Neoplastic , Glioma , Genetics , Pathology , MicroRNAs , Genetics , Oligoribonucleotides, Antisense , Genetics , Pharmacology , PTEN Phosphohydrolase , Metabolism , TransfectionABSTRACT
Endothelial progenitor cells(EPC),which have the capacity to differentiate into mature endothelial cells,have been found to home to and incorporate into the angiogenic vasculature of growing tumors with high specificity once mobilized into the circulation.Yet the mechanism still remains unclear.EPC have potential as spatial-specific delivery vehicles.Thus,further studies on the mechanisms of tumor neovascularization and tumor therapy in glioma using genetically engineered EPC as angiogenesis-selective vectors will be of help to explore the potential in the basic and clinical application of EPC in(glioma.)
ABSTRACT
The PIWI family members are defined as conserved PAZ and PIWI domains and play important roles in stem-cell self-renewal,spermatogenesis,RNA interference and translational regulation.Recent researches have showen that some PIWI are specifically expressed in tumors and associated with tumor development and growth.PIWI is likely to be a new significante index for tumor diagnose and prognosis,the feasibility of PIWI acting in tumor gene theropy could be in vestigated by studying the mechanism of PIWI expression and regulation.
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of connexin gene (Cx43) in the suppression of C6 glioma.</p><p><b>METHODS</b>Cx43 gene depleted parental C6 rats (control group) and C6 cells transfected with Cx43 cDNA (transfection group) were implanted into the right caudate nucleus of SD rats. Rats bearing cerebral C6 gliomas were treated with Cx43 cDNA (treatment group) with another group treated with empty vector (empty vector group) serving as control. The general manifestation, survival time, MRI dynamic scanning and histopathological changes in all rats were observed. Cx43 mRNA and its protein were examined by in situ hybridization and immunohistochemistry. Proliferation activity was monitored by the average number of AgNOR stain. Cell apoptosis was examined by the Tolt-mediated x-duTP nick end labeling (TUNEL) method.</p><p><b>RESULTS</b>All rats in the control and empty vector groups died of cerebral glioma within 3 weeks after implantation of C6 cells. Six in the transfection group and 8 in the treatment group were alive beyond 120 days with complete disappearance of the tumor foci, except one in this group having some residue of tumor. In the glioma of transfection and treatment groups, Cx43 gene expression was up-regulated, proliferation activity reduced while the apoptotic cells did not increase.</p><p><b>CONCLUSION</b>The development of glioma is greatly suppressed by the transfection of Cx43 gene, which has great effectiveness in rats bearing cerebral malignant gliomas. This could become a target of choice in the gene treatment of malignant gliomas.</p>
Subject(s)
Animals , Male , Rats , Brain Neoplasms , Mortality , Therapeutics , Connexin 43 , Genetics , Therapeutic Uses , Disease Models, Animal , Genetic Therapy , Glioma , Mortality , Therapeutics , Neoplasm Transplantation , Rats, Sprague-Dawley , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To investigate telomerase activity and expression of hTR and hTERT in human neuroepithelial tumors for exploring new strategy for clinical diagnosis and treatment.</p><p><b>METHODS</b>Telomerase activity was detected by modified TRAP method and the expression of hTR and hTERT was measured by RT-PCR method in 65 human neuroepithelial tumors, respectively.</p><p><b>RESULTS</b>The positive rates of telomerase and hTERT were 61.54% and 70.77% respectively in human neuroepithelial tumors, and the positive rate and their level of expression were correlated with the degree of malignancy of tumors positively.</p><p><b>CONCLUSIONS</b>Telomerase activity and hTERT are significantly correlated with the degree of malignancyin human neuroepithelial tumors. hTERT may play a key role in the regulation of telomerase activity.</p>
Subject(s)
Humans , DNA-Binding Proteins , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Neoplastic , Neoplasms, Neuroepithelial , Genetics , Telomerase , Genetics , MetabolismABSTRACT
Objective To measure anatomical parameter of internal carotid artery in petrous bone, and provide anatomical basis for operations Methods The anatomical study of the internal carotid artery (ICA) in petrous bone and related landmarks was studied in 15 adult cadaver brain specimens under the microscope Results The petrous segment of the ICA can be divided into ascending,bend and horizontal portion The great superficial petrous nerve was the best landmark through which the petrous segment of ICA can be exposed in operations. Conclusions If we are familiar with the anatomy of the petrous segment of the ICA and related landmarks,the petrous segment of the ICA can be protected very well in operations with less complications and better prognosis.
ABSTRACT
Objective: To obtain anatomical parameters for surgery of the cavernous sinus. Methods:The anatomical study of nerves and triangles of the cavernous sinous was made in 15 adult cadaver brians by microanatomical methods. Results: Ten triangles of the cavernous sinus and 3rd cranial nerve, 4th cranial nerve in each cavernous sinus were studied. It was discovered that almost all content could be shown through Hakuba triangle and Parkinson triangle. Conclusion: The anatomical parameters of cavernous sinus is obtained, it is useful for surgical treatment of cavernous diseases. [